Table 1.
Thermodynamic parameters of the binding between p41 and the WT and R21A variants of the Spc-SH3 domain at 25°C measured by ITC1
| pH | Protein | Kb (×10-3 M-1) | ΔHb (kJ mol-1) | p | TΔSb (kJ mol-1) | ΔGb (kJ mol-1) |
| 7.0 | R21A | 18.1 ± 1.9 | -58.7 ± 2.5 | 0.06 ± 0.05 | -34.4 | -24.3 |
| WT | 5.2 ± 1.2 | -45.4 ± 6.3 | 0.01 ± 0.12 | -24.2 | -21.2 | |
| Abl-SH3 | 323 | -91.6 | 0.1 | -60.2 | -31.4 | |
| 3.0 | R21A Spc-SH3 | 18.2 ± 2.0 | -51.3 ± 1.7 | -0.29 ± 0.08 | -27.0 | -24.3 |
| WT Spc-SH3 | 5.8 ± 0.8 | -43.9 ± 3.3 | -0.07 ± 0.11 | -22.4 | -21.5 | |
| Abl-SH3 | 210 | -93.3 | - | -62.9 | -30.4 | |
1The similar parameters for the Abl-SH3 domain are also shown for comparison and are taken from reference 16. The errors in each parameter were estimated from 95% confidence intervals as determined from the fittings of the binding isotherms.