TABLE 1.

ITC data for titration of SDS into solutions of TII27 and TNfn3

Protein*	Region	[SDS]aq (mM)†	N‡	Δ (kcal mol−1)§	αΔH (kcal mol−1 mM−1)¶
TNfn3	Peak I	4.9 ± 0.1	16.9 ± 1.5	0.117 ± 0.072	7.6 ± 1.8
TNfn3	Peak II	5.7 ± 0.1	40.2 ± 1.8	−0.054 ± 0.017	−2.4 ± 0.4
TII27	A*	‖	‖	−0.102 ± 0.020	−2.1 ± 0.2
TII27	Peak I	4.2 ± 0.4	14.8 ± 5.2	**	**
TII27	Peak II	5.1 ± 0.3	44.0 ± 3.0	0.010 ± 0.015	−4.8 ± 0.1

^*All experiments in 10 mM Tris pH 8.0 at 22°C. Errors from the linear fits in Fig. 5.

^†Intercept from plot of the position of the peak (mM SDS) versus protein concentration.

^‡Slope from plot of the position of the peak (mM SDS) versus protein concentration.

^§Intercept from plot of the height of the peak (kcal/mol) versus protein concentration.

^¶Slope from plot of the height of the peak (kcal/mol) versus protein concentration (mM).

^‖Not determined because no well-defined minimum available (Fig. 4 B and text).

^**No significant linear correlation observed (Fig. 5 C).