FIGURE 3.

Schematic representation of single-cell electroporation geometry. The pulled end of the capillary is close to the targeted cell and is positioned at ∼45° with respect to the dish normal. The full capillary is much longer than shown. Its distal end is placed in a vial of buffer where an electrode that delivers electrical pulse is found. The capillary is filled with the same conducting buffer (not illustrated) that surrounds the cell. The electrical circuit is completed with a ground electrode placed far away from the targeted cell.