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Fluorescence properties of Trp mutants of MscL
| Mutant | λmax (nm) in di(C18:1)PC | Distance along bilayer normal from L69 (Å) |
|---|---|---|
| A20W | 329.3 ± 0.2 | 24.3 |
| I23W | 325.2 ± 0.1 | 20.1 |
| L30W | 327.6 ± 0.1 | 11.6 |
| V31W | 323.9 ± 0.1 | 11.6 |
| F34W | 326.2 ± 0.4 | 7.1 |
Wavelengths of maximum fluorescence emission intensity (λmax) were obtained by fitting fluorescence spectra recorded in di(C18:1)PC to Eq. 1 for a skewed Gaussian. Distances along the bilayer normal were calculated as described in the text.
