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Scale diagram showing the relationship of the six fixed-charge amino acids close to H322 within the central cavity of LacY. Also shown are the single mutations at each of these positions and their effects on LacY transport function. NS, no symport; U, uniport; NU, no uniport; UL, lactose uniport; DS, downhill H+ -lactose symport. The shaded areas delineate the planar trapezoids between K319/R302/E325/E269 KREE en face and K319/D240/D237/E325 KDDE perpendicular.
