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. 2000 May 23;97(11):5907–5912. doi: 10.1073/pnas.97.11.5907

Figure 1.

Figure 1

Human CLU/XIP8 interacts with Ku70 by yeast two-hybrid analyses. (Left) Limited deletion analyses showed that the C terminus of CLU/XIP8 interacted with the C terminus of Ku70. Nineteen separate CLU/XIP8 clones, with 15–1 (C-terminal 120 aa) being the shortest and 73–1 the longest, bound to the C terminus of Ku70. A Ku70 deletion mutant, eliminating 276 bp, was created by StuI digestion (8). The ER-hydrophobic signal is indicated by ####. NLSs are indicated by *. (Right) CLU/XIP8 clones, 15–1 and 73–1, were used to characterize the interaction between CLU/XIP8 and Ku70. Positive interacting yeast colonies were grown on histidine(−) medium and monitored for β-galactosidase activity (blue spots, shown). Ku70 StuI deletion mutant, Ku80, p53, cdk2, lamin B, and SNF-1 did not interact with CLU/XIP8 clones, 15–1 or 73–1.