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. Author manuscript; available in PMC: 2007 Apr 20.
Published in final edited form as: Parasitology. 2006 Jan;132(Pt 1):57–65. doi: 10.1017/S0031182005008772

Fig. 1.

Fig. 1

PCR-based identification of Trypanosoma cruzi lineages in faeces and culture isolates of naturally infected T. infestans. (A) Identification of TCI and TCII by SL-DNA PCR. TCI is identified from a culture isolate of specimen IG-41 and TCII from faeces of specimen P-15. DG-100-F and DG-100-10 are faecal and culture samples from a same triatomine, which revealed a dual TCI+TCII infection in faeces (DG-100 F) but only TCII in culture (DG-100-10). (B) Distinction between TCIId and TCIIb or TCIIe by 24Sα rDNA-PCR. TCIId is identified in a culture isolate from specimen SI-2-6 (125 bp + 140 bp amplicons), whereas TCIIb or TCIIe are detected in the culture isolate of DG-100-10 (140 bp product alone). (C) Differential amplification of TCIIb and TCIIe by A10 and 18S rDNA-PCR. TCIIb control strain shows only amplification of 18S rDNA genes. In contrast, TCIIe control strain and culture isolate from specimen DG-100-10 show amplification of both markers. T. cruzi control strains: TCI, T. cruzi I (X-10); TCllb, T. cruzi IIb (Tu 18); TC IId, T. cruzi IId (Mn CL2); TCIIe, T. cruzi IIe (CL-Brener). M1: 100 bp ladder DNA molecular weight marker, M2, 50 bp ladder DNA molecular weight marker; 3% agarose gel electrophoresis stained with ethidium bromide.