Table 1.

PCR-based identification of Trypanosoma cruzi lineages in faeces and culture isolates from triatomine bugs: DNA targets, application, primer sets and amplicon expected sizes in base pairs

	DNA Target	Application	Primer sets	TCl	TClla	TCllb	TCllc	TClld	TClle
I	Spliced leader intergenic region	Distinction of TCl from TClla, llc & TCllb, lld, lle	TCC-TC1-TC2a	350	n/a	300	n/a	300	300
			TCac-UTCCb	157	200	150	200	150	150
II	24S alpha rDNA	Distinction between TClla & TCllc or between TClld & TCllb, lle	D71-D76b	125	135	140	125	125+140	140
III	18S rDNA A10 fragment	Distinction between TCllb & TClle	V1-V2c	175	155	165	165	165	n/ac,d
			P3-P6c	n/a	657	n/a	657	657	657

I to III: Work flow for lineage identification. Boxed amplicon sizes denote the PCR findings of this survey.

n/a: no amplification.

^aSouto et al. 1996.

^bHeminested PCR (Burgos et al. 2005).

^cBrisse et al. 2001.

^dMost TClle isolates from northwestern Argentina amplify a 165bp 18S rDNA fragment.