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. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Anal Biochem. 2006 Dec 22;362(2):193–200. doi: 10.1016/j.ab.2006.12.035

Figure 2.

Figure 2

An image of PCR products following separation on a 2% agarose gel and imaged with ethidium bromide. Lane MW is a 100 base pair DNA ladder in which the 1,000 bp and 500 bp bands appear twice as intense. The wild-type and deleted mtDNA products are 411 and 429 bp, respectively. Lanes 1–4 contained deleted mtDNA and lanes 5–8 contained wild-type mtDNA. Lanes 1 and 5 contained primers F1 and R1, lanes 2 and 6 contained primers F1 and R2, lanes 3 and 7 contained primers R1 and R2 and lanes 4 and 8 contained all three primers but neither template.