Table 1.
Primers and molecular beacons
| Primers | Position | Sequence |
|---|---|---|
| S1 | 7743–7763 | 5′-CTAACATCTCAGACGCTCAGG |
| S2 | 8290–8310 | 5’-AGTTAGCTTTACAGTGGGCTC |
| S3 | 15,909–15,928 | 5′-CCGGTTTACAAGACTGGTGT |
| F1 | 7798–7820 | 5′-CATCCTAGTCCTCATCGCCCTCC |
| R1 | 8186–8209 | 5′-GGGCATGAAACTGTGGTTTGCTCC |
| R2 | 15,727–15,749 | 5′-GAATGAGGAGGTCTGCGGCTAGG |
|
| ||
| Molecular Beacons | Position | Sequence |
|
| ||
| MB1a | 8072–8092 | FAM-5′-CCAGCGGCCTAATGTGGGGACAGCTCACGCTGG-BHQ1 |
| MB2a | 15,534–15,553 | HEX-5′-CCAGCGCTTGATGTGGGGAGGGGTGTCGCTGG-BHQ1 |
a
The stem sequence is shown in bold