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. Author manuscript; available in PMC: 2007 Apr 20.
Published in final edited form as: J Biol Chem. 2006 Oct 16;281(50):38472–38477. doi: 10.1074/jbc.M607577200

FIGURE 2. Deletion of the 53BP1 N terminus or its conserved (S/T)Q sites abolishes the repair function of 53BP1.

FIGURE 2

A, schematic representation of the 53BP1 deletion mutants that gradually remove the 15 conserved N-terminal (S/T)Q sites. NLS, nuclear localization sequence. B, average number of residual P-H2AX foci 27 h after 2 Gy in superconfluent wild-type (wt) MEFs, 53BP1-null (k.o.) MEFs, or cells stably reconstituted with wild-type (FL) or various mutants of human 53BP1. The 15AQ mutant has all 15 conserved (S/T)Q sites replaced with AQ sites. The error bars represent the S. E. of triplicate samples.