Figure 5. Majority of beaded dendrites appear to be interneurons.

A: Control slice from GAD67-GFP animal processed for MAP2 immunoreactivity (red). This representative example shows a low density of GFP-positive neurons (green) in the hippocampal CA1 region. B: Higher power image of control slice on left. C: MAP2-positive beaded dendrites (arrowheads) observed immediately following NMDA exposure in 0 Ca²⁺. C’: GFP fluorescence in the same preparation illustrated in C, showing co-localization with MAP2 in dendrite beads (arrowheads) D: Representative GFP-positive neuron observed under control conditions. E: Extensive dendritic beading in a GFP-positive neuron, observed immediately following NMDA exposure in 0 Ca²⁺. F: Mean data illustrating that in CA1 stratum radiatum most GFP-positive processes were beaded under these stimulation conditions. G: GFP-positive dendrites also accounted for the majority of beaded dendrites detected with MAP2 under these conditions (n=6). Scale bars A: 50μm; B, D, E: 10μm; C: 5μm.