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. Author manuscript; available in PMC: 2008 Feb 16.
Published in final edited form as: J Mol Biol. 2006 Dec 1;366(2):574–585. doi: 10.1016/j.jmb.2006.11.068

Figure 7.

Figure 7

(a) Overlay of 2D 1H-15N HSQC spectra upon titration of V7R with di-C4-PI(4,5)P2 [50 μM, 35 °C; di-C4-PI(4,5)P2:MA = 0:1 (black), 1:1 (red) , 2:1 (grey), 4:1 (magenta), 8:1 (green), 16:1 (blue)]. (b) 15N NMR chemical shift titration data, which fit to 1:1 binding isotherms (Kd = 261 ± 18 μM). Representative 13C-edited/12C-double-half-filtered NOE data showing unambiguously assigned intermolecular NOEs between (c) Leu-21 and di-C4-PI(4,5)P2, and (d) Ile-34 and Leu-85 to the myristate group. Solid and dashed lines denote 1H-12C breakthrough doublets and intermolecular NOE peaks, respectively.