Table 1.
Comparison of MSP-1 Mad20 sequences with the sequence detected in the Mad20 region of MR, showing the S49-3 M20-150 clone to be the closest match
| Kn: MR consensus sequence | (0) ...CAAAG-GGTTCAGTT-ACTTCAGGT-GGTTCAGGT-GGTTCAGGT ...| 0 | |
| 0 substitutions in repeat region shown, 0 substitutions in the upstream Block 1 sequence, same Block 2 length | |
| Kn: S49-3 M20-150 clone | (0) ...*****-*********-*********-*********-********* ...| 0 | |
| 2 substitutions in repeat region shown | |
| Kn: M20-190 | (nd) ...***GT-*********-*********-*********-********* ...| 0 | |
| 3 substitutions in repeat region shown | |
| Tz: HN3 | (0) ...*****-*******G*-*********-*********-**C****T*...| 1 | |
| Tz: HN2 | (0) ...*****-*******G*-G********-*C*******-********* ...| 3 | |
| Ti: 828 | (0) ...*****-*********-*********-*******T*-AC******* ...| 2 | |
| Sg: Bandia | (3) ...*****-*********-G********-*********-**C****T* ...| 2 | |
| Pfant195, 195A, Hd: Gp185, Ti : 837, 822b, 827, 808 | (0) ...*****-*********-G********-*********-**C****T* ...| 2 | |
| Vt: 195RR, Ti 806 | (1) ...*****-*********-G********-*********-**C****T* ...| 3 | |
| PfANT1, AF218248, Tz: HN627, Ti: MSA1aJ | (0) ...***GT-*********-*********-*********-*******T* ...| 0 | |
| Kn: M20-190 | (nd) ...***GT-*********-*********-*********-*******T* ...| 3 | |
| Tz: IFA9-10 | (3) ...***GT-*********-*********-*********-*******T* ...| 4 | |
| Tz: IFA10 | (5) ...***GT-*********-*********-*********-*******T* ...| 5 | |
| 4 substitutions in repeat region shown | |
| Hd: HB3a | (0) ...*****-*********-G********-********T-**C****T* ...| 1 | |
| So: PaloAlto | (3) ...****A-*********-G********-*********-**C****T* ...| 1 | |
| Ti: 842, Tz: 2/M1 | (0) ...***GT-*********-********-*********-**C****T* ...| 1 | |
| Ti: 822a | (0) ...***GT-*********-*********-*********-**C****T* ...| 2 | |
| Kn: M20-180 | (nd) ...***GT-*********-*********-*********-**C****T* ...| 2 | |
| 5 substitutions in repeat region shown | |
| Tz: HN624 | (0) ...***GT-*********-*********-*******T*-AC******* ...| 0 | |
| Ti: 834a | (1) ...***GT-*********-*********-*******T*-AC******* ...| 1 | |
| Ti: 947, 836, 807, 815, 835b, PNG: FC-27 | (0) ...***GT-*********-*********-*******T*-AC******* ...| 1 | |
| Pfp190G1 | (0) ...***GT-*********-*********-*******T*-******GT* ...| 1 | |
| Kn: M20-200 | (nd) ...****n-*******G*-GG*****T*-*C*******-********* ...| 6 | |
| > 5 substitutions in repeat region shown | |
| D43960 | (0) ...C*GGT-*C*****G*-*********-**C****T*-*C******* ...| 1 | |
| Tz: IFA11 | (0) ...C*GGT-*********-G********-**C****T*-*C******* ...| 5 | |
| Kn: M20-170 | (nd) ...C*GGT-*C*****G*-GG*****T*-*C*******-********* ...| 3 | |
| Tz: IFA125 | (1) ...C*GGT-*C*****G*-GG*****T*-*C*******-********* ...| 3 | |
| Tz: IFA12 | (0) ...C*GGT-*C*****G*-GG*****T*-*C*******-********* ...| 3 | |
| Kn: M20-180 | (nd) ...C*GGT-*C*****G-GG*****T*-*C*******-********* ...| 4 | |
| 3 substitutions and one deleted repeat in the repeat region shown | |
| Ti: 843a | (0) ...*****-*********-G*****A**-*******T*---------- ...| 4 | |
Forty-five available Mad20 (M20) sequences were compared with the consensus for the MR sequences (see Fig. 1). Only the S49-3 M20-150 clone was an exact match to these characteristics. The other sequences were grouped by the number of substitutions in the repeat region involved in the recombination (sequence shown). The other sequences had at least two substitutions in the repeat region shown. Subtypes were defined by the count of substitutions in the MSP-1 5′ Block1 region when compared with the MR-150 S13A clone (shown in the 5′ parentheses). The value in bars shows the difference in Block 2 repeat unit copy number vs. the MR-150 S13A clone, indicating differences in length. The sequences are identified by geographic origin when available (Ti, Thai; Kn, Kenya; PNG, Papua New Guinea; Tz, Tanzania; Sg, Senegal; So, South America; Hd, Hondoras; Vt, Vietnam) and by key characters in their Genbank accession number or identification number used for sequences reported in this study. Not enough data, nd. Unassigned nucleotide, n.