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. Author manuscript; available in PMC: 2007 Apr 20.
Published in final edited form as: Mol Biochem Parasitol. 2005 Nov 15;146(1):30–37. doi: 10.1016/j.molbiopara.2005.10.015

Fig. 1.

Fig. 1

Generation of a transgenic β-galactosidase-expressing Plasmodium berghei parasite. (A) Insertion strategy to place lacZ expression under control of the CSP promoter. The targeting vector contains the CSP promoter region (pale grey bar), the lacZ coding region (grey box) and the 3′UTR of the DHFR/TS gene (dark grey bar) to allow for proper transcript processing, together with the positive selectable marker dhfr/ts (black box). The targeting vector was linearized within the CSP 5′UTR by Acc65I [A]. Test primers are indicated by arrows and expected fragments are shown as lines. (B) Integration-specific PCR confirms the predicted transgenic locus. Note that the primer combination ‘test2’ is indicative of an integration event.