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. Author manuscript; available in PMC: 2007 Apr 20.
Published in final edited form as: Mol Biochem Parasitol. 2005 Nov 15;146(1):30–37. doi: 10.1016/j.molbiopara.2005.10.015

Fig. 3.

Fig. 3

Detection of transgenic sporogonic and pre-erythrocytic Plasmodium stages expressing β-galactosidase. (A) Midgut oocysts of infected Anopheles mosquitoes at day 13 post feeding. All oocysts can be stained with X-Gal after fixation and permeabilization. Scale bar is 200 μm. (B) X-Gal staining of an infected salivary gland after permeabilization. Scale bar is 100 μm. (C) Transgenic sporozoites express β-galactosidase (left panel) and perform gliding locomotion indistinguishable from WT sporozoites (right panel). Scale bar is 5 μm. (D) Infected heptocytes can be stained with X-Gal after fixation and permeabilization. Shown is a 48 h liver stage. Scale bar is 20 μm.