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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Steroids. 2006 Dec 18;72(2):117–123. doi: 10.1016/j.steroids.2006.11.006

Figure 4.

Figure 4

Gβγ and Gαs have opposite effects on intracellular cAMP in mouse oocytes. (A) Follicle-enclosed mouse oocytes were injected with either buffer (Mock, circle) or cRNAs encoding either Gαs by itself (square) or Gαs plus Gβ and Gγ (triangle). After an overnight incubation, oocytes were gently denuded and GVBD determined at the indicated times. Approximately twenty oocytes from each set of injections were used. Percent GVBD is shown in the graphs, and the relative intracellular cAMP levels from the same batch of oocytes at five hours are indicated on the right. Western blot analysis of injected oocytes demonstrates equal expression of Gαs (B). (C & D) The same experiments as shown in A & B, only oocytes were injected with cRNA encoding constitutively activated Gαs (Gαs-ca) instead of wild-type Gαs. Notably, here Gαs-ca increased cAMP 12-fold above mock-injected cells, while cAMP in oocytes co- expressing Gβγ with Gαs-ca were only 3.8-fold above mock-injected cells. All experiments were repeated at least twice with similar results.