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. Author manuscript; available in PMC: 2007 May 1.
Published in final edited form as: Biol Psychiatry. 2006 Nov 1;61(9):1049–1061. doi: 10.1016/j.biopsych.2006.08.005

Figure 7. De novo synthesis of STEP in the lateral amygdala following fear conditioning.

Figure 7

(A) Tissue punches taken from LA at 5, 10, 15, 20 and 60 min after fear conditioning or from control animals (no tone or shock, lane C) were analyzed by SDS-PAGE and processed for immunoblotting with anti-STEP (upper panel). (B) Rats were exposed to either shock or tone alone and punches were taken from the LA at 10 minutes, when STEP levels are at their maximum after fear conditioning (n = 3 independent trials with 3 rats per treatment). Note the increase in both STEP isoforms after fear conditioning. (C) pERK1/2 levels were determined after fear conditioning (upper panel). (D) Rats were injected intraperitoneally with DMSO (vehicle), cycloheximide (protein synthesis inhibitor) or SL327 (MEK inhibitor) 1 hr prior to fear conditioning. Tissue punches were again processed at 10 minutes for immunoblotting with anti-STEP antibody (upper panel). These experiments were repeated in triplicate with p<0.02 for both inhibitors. All blots were reprobed with anti-ERK2 antibody to allow for comparisons of loading levels (A, B, C & D, all lower panels).