Table 2.
HLA locus | Number of alleles | IFN-γ
|
IL-4
|
|||||
---|---|---|---|---|---|---|---|---|
Median secretion value (Q1, Q3) pg/ml | Allele p-valueb,c | HLA-DR-adjusted p-valued | Number of alleles | Median secretion value (Q1, Q3) pg/ml | Allele p-valueb,c | HLA-DR-adjusted p-valued | ||
Overall | 612 | 27.7 (1.8, 109.4) | 568 | −0.6 (−7.1, 6.2) | ||||
HLA-DQB1 | ||||||||
*0201 | 82 | 29.0 (6.9, 111.8) | 0.31 | 0.14 | 77 | 2.6 (−4.6, 10.7) | 0.06 | 0.80 |
*0202 | 47 | 15.7 (−3.0, 78.6) | 0.28 | 0.92 | 44 | −0.7 (−7.0, 6.3) | 0.69 | 0.58 |
*0301 | 117 | 23.5 (−2.9, 95.7) | 0.36 | 0.98 | 110 | −0.6 (−7.8, 4.9) | 0.42 | 0.58 |
*0302 | 61 | 39.6 (5.7, 121.8) | 0.30 | 0.52 | 55 | 0.8 (−6.4, 7.9) | 0.41 | 0.81 |
*0303 | 24 | 27.6 (3.2, 174.8) | 0.67 | 0.57 | 20 | −2.3 (−9.1, 9.9) | 0.80 | 0.44 |
*0402 | 17 | 23.9 (3.3, 55.5) | 0.51 | 0.59 | 15 | −1.4 (−8.0, 17.9) | 0.85 | 0.52 |
*0501 | 70 | 46.6 (3.5, 106.8) | 0.30 | 0.28 | 71 | −0.9 (−7.3, 5.3) | 0.60 | 0.24 |
*0502 | 10 | 74.6 (−1.6, 157.3) | 0.74 | 0.50 | 7 | −0.4 (−6.6, 7.8) | 0.68 | 0.56 |
*0503 | 15 | 17.4 (0.7, 70.2) | 0.61 | 0.76 | 13 | −1.2 (−7.2, −0.2) | 0.44 | 0.81 |
*0602 | 85 | 23.9 (−3.0, 122.2) | 0.70 | 0.33 | 77 | −0.7 (−9.3, 5.8) | 0.50 | 0.59 |
*0603 | 41 | 33.5 (−5.9, 65.3) | 0.20 | 0.17 | 42 | −3.0 (−9.5, 2.4) | 0.10 | 0.65 |
*0604 | 30 | 13.6 (−2.9, 142.1) | 0.58 | 0.59 | 26 | 2.1 (−5.7, 11.1) | 0.29 | 0.02 |
All other allelese | 13 | 114.8 (17.6, 15.0) | 0.04 | 0.02 | 11 | 0.8 (−16.2,15.0) | 0.96 | 0.84 |
HLA-DPB1 | ||||||||
*0101 | 46 | 26.9 (11.9, 111.8) | 0.27 | 0.81 | 45 | −1.2 (−8.7, 11.3) | 0.82 | 0.46 |
*0201 | 74 | 32.9 (1.9, 111.8) | 0.53 | 0.28 | 67 | 1.7 (−4.1, 11.1) | 0.05 | 0.02 |
*0301 | 65 | 25.5 (3.5, 121.8) | 0.78 | 0.81 | 58 | −0.7 (−8.9, 7.4) | 0.63 | 0.48 |
*0401 | 233 | 27.4 (−0.7, 106.8) | 0.36 | 0.32 | 213 | 0.2 (−7.2, 6.3) | 0.80 | 0.63 |
*0402 | 74 | 25 (−0.3, 114.8) | 0.61 | 0.64 | 74 | −1.7 (−8.5, 5.6) | 0.17 | 0.28 |
*0501 | 18 | 29.9 (0.1, 106.8) | 0.95 | 1.00 | 16 | −2.6 (−7.8, 2.2) | 0.25 | 0.21 |
*0601 | 13 | 14.6 (5.0, 50.9) | 0.65 | 0.54 | 11 | 0.3 (−4.6, 4.6) | 0.66 | 0.68 |
*1101 | 11 | 9.9 (−5.7, 29.6) | 0.18 | 0.23 | 10 | 0.5 (−2.7, 12.4) | 0.33 | 0.64 |
*1301 | 13 | 28.0 (6.6, 132.3) | 0.98 | 0.61 | 13 | −2.1 (−8.7, 0) | 0.10 | 0.09 |
*1401 | 11 | 29.3 (−5.4, 127.2) | 0.95 | 0.86 | 11 | 5.3 (−2.0, 13.5) | 0.27 | 0.17 |
*1501 | 7 | 7.6 (−4.7, 164.2) | 0.87 | 0.88 | 6 | 4.9 (−1.0, 17.9) | 0.28 | 0.36 |
*1701 | 7 | 17.4 (0.1, 78.6) | 0.97 | 0.75 | 7 | −0.6 (−6.1, 6.3) | 1.00 | 0.82 |
All other allelese | 40 | 44.4 (3.2, 148.0) | 0.27 | 0.23 | 37 | −2.9 (−5.5, 4.7) | 0.35 | 0.44 |
Q1, Q3 represent the first and third quartiles, respectively.
Age-adjusted global p values are as follows: HLA-DQB1 IFN-γ, 0.46; HLA-DPB1 IFN-γ 0.93; HLA-DQB1 IL-4, 0.70; HLA-DPB1 IL-4, 0.36. Age and HLA-DRB1-adjusted global p values are as follows: HLA-DQB1 IFN-γ, 0.18; HLA-DPB1 IFN-γ, 0.95; HLA-DQB1 IL-4, 0.49; HLA-DPB1 IL-4, 0.28. Suggestive findings (p < 0.10) are shown in bold.
Mean value of antigen stimulated cells minus mean value of control cells.
Linear regression analysis, accounting for the design variable age. Genotypes were modeled as ordinal variables with values ranging from 0 to 2, reflecting the number of copies possessed by an individual. Due to data skewness, all secretion values were rank-transformed.
Comparing genotype of interest to all other genotypes combined.
Analyses adjust for age and the HLA-DRB1*01, *02, *03, *04, *05, *06, *07, *08, and *09 alleles.
“Other” includes the following HLA-DQB1 alleles: *0203 (n = 2), *0304 (n = 1), *0306 (n = 2), *0601 (n = 4), *0608 (n = 1), *0609 (n = 1), *0614 (n = 2); and HLA-DPB1 alleles: *0202 (n = 4), *0901 (n = 6), *1001 (n = 6), *1601 (n = 4), *1901 (n = 4), *2001 (n = 3), *2101 (n = 1), *2301 (n = 3), *2401 (n = 1), *2601 (n = 2), *3001 (n = 1), *5001 (n = 1), *5101 (n = 1), *6301 (n = 1), *6601 (n = 2), respectively.