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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: J Neuroimmunol. 2006 Oct 2;181(1-2):165–176. doi: 10.1016/j.jneuroim.2006.08.007

Fig. 6. Reactivity of Streptococcal Proteins with Anti-PK Antibody.

Fig. 6

(A) Proteins from the M serotypes 18, 12, 6 and 3 (with designated strain numbers) were fractionated into cytosolic (cyt) and extracellular membrane associated fractions (ext) (10 μg total protein/lane) and immunoblotted using anti-PK antibody. Purified PK (1 μg) was loaded in the first lane (left). The lower panel in A shows a control blot under identical conditions but with goat serum (1:200 dilution) in place of the primary antibody (film exposed time is 10 fold longer than in upper panel). (B) Immunoblots of purified PK (0.5 and 1.0 μg) using anti-M5, -M6, and -M24 antibodies. Enolase was also screened for reactivity under the same conditions used for PK. The two panels in the figure labeled Con are controls using normal rabbit serum (diluted 1:200) in place of the primary antibodies (film exposure times are 30 fold longer than in the upper panels). (C) Lysate proteins (100 μg/lane) from the indicated rat brain tissues were immunoblotted with anti-M24 antibody (left panel) and anti-PK antibody (right panel). The panels immediately below each are controls showing identical blots but normal rabbit serum (1:200 dilution) and normal goat serum (1:200 dilution) were used in place of the primary antibodies anti-M-24 and Anti-pk, respectively (film exposure times are 7 fold longer than in the upper panels).