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Specificity of immunohistochemical staining of fibulin-1. Adjacent sections from a serous ovarian carcinoma were treated with 2 μg/ml of the 3A11 anti-human fibulin-1 MAb alone (a), with a 100-fold excess of pure fibulin-1 (b), or with an irrelevant antibody (2 μg/ml UPC 10; (c)). Counterstaining was with hematoxylin. Bar, 50 μm.
