Fibulin-1 RNA expression in adjacent frozen sections of one serous ovarian carcinoma (a to c) and two serous cystadenomas (d to i). In situ hybridization was performed without counterstaining with fibulin-1 antisense probe (b, e, and h) and with sense probe (a, d, and g) as negative control. Adjacent sections were stained with anti-fibulin-1 3A11 antibody (c and f) and with hematoxylin and eosin (i). Bar, 50 μm.