Figure 4.

Western blot analysis of CCR5 protein in normal and lesioned neonatal rat hippocampus. Samples were prepared from left (L) and right (R) hippocampus of animals that received a right intrahippocampal injection of 10 nmol of NMDA or PBS 32 hours earlier and from unlesioned P8 animals were analyzed, together with a sample from adult rat spleen (included as a positive control; see Materials and Methods). A single immunoreactive band of the expected size (approximately 41 kd) was detected in all samples tested. There was a robust increase in CCR5 content in the NMDA-lesioned hippocampus in comparison with samples from normal P8 and PBS-injected animal; a less pronounced increase was also evident in the left hippocampus of NMDA-lesioned animals.