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. 1998 Nov;153(5):1551–1560. doi: 10.1016/S0002-9440(10)65743-4

Figure 1.

Figure 1.

Expression of the four septins in AD and control brains. a: RT-PCR analysis of the human septin genes expressed in the hippocampus. Poly(A)+ RNA (∼10 ng) from human hippocampal regions was reverse-transcribed and subjected to PCR using primer sets for human Nedd5, Diff6, H5, and hCDC10, and for β-actin as an internal control. Representative results for AD brains (lanes 1–3), age-matched (lanes 4–7, 10) and young (lanes 8, 9) control brains, and a negative control without template cDNA (NC) are shown. b: Characterization of the four anti-septin antibodies used in this study. Each antibody (6 and 11 for Nedd5, H5C-1 for H5, and D6C-1 for Diff6) was tested by immunoblotting of lysates from mouse embryos (at gestational day 13.5) and a normal human hippocampus. Each lane contained 25 μg of total protein. Immunoreactive bands (arrowheads) were abolished when antibodies preadsorbed with the corresponding antigens were used. c: Immunoblot of the hippocampal lysates of AD and control brains. A representative blot showing Nedd5 (detected by antibody 11) in brains from AD patients (lanes 1–3), age-matched controls (lanes 4–7, 10) and the young individuals (lanes 8, 9). Each lane contained 25 μg of total protein.