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. 1999 Jan;154(1):45–51. doi: 10.1016/S0002-9440(10)65249-2

Figure 1.

Figure 1.

Cryostat sections from active demyelinating MS lesions (case S59/S116) and chronic active demyelinating MS lesions (case S276/S283). A: Histochemical staining with the neutral lipid marker Oil Red O (ORO) in an active demyelinating lesion. Abundant lipid-filled ORO-positive perivascular and parenchymal macrophages are distributed throughout the demyelinated lesion (arrowheads). B: Immunohistochemical staining with the macrophage marker KP-1 (CD68) in an active demyelinating MS lesion. Strong immunoreactivity for KP1 was detected in both foamy perivascular and parenchymal macrophages (arrowheads). C: A higher magnification shows the CD68-immunoreactive macrophages (arrowheads). D: Immunohistochemical staining with KP1 MAb in a chronic active demyelinated MS lesion. Fewer KP-1-positive parenchymal macrophages were detected in the lesion center, whereas in the hypercellular rim abundant KP1 immunoreactivity was present in macrophages (arrowheads). LC, lesion center; HR, hypercellular rim. E: ORO staining of a chronic active demyelinating lesion. Degraded lipid was detected in the parenchymal foamy macrophages in the hypercellular rim (arrowheads). F: Immunohistochemical staining with a GFAP-specific PAb. Hypertrophic, reactive astrocytes were detected in the lesion center and the hypercellular rim surrounding the demyelinated region (arrowheads). Magnification, ×100 (B and D), ×200 (A, E, and F), and ×400 (C).