Table 1.
Monoclonal Antibodies and Antisera Used in this Study
| Molecule/antibody | Clone | Pretreatment, Antibody dilution | Source |
|---|---|---|---|
| CD117 (c-kit) | polyclonal | MW, 1:400 | Santa-Cruz Biotechnology, Santa Cruz, CA |
| CD34 | HPCA-1 | Pepsin, 1:50 | Becton-Dickinson, Mt View, CA |
| α-smooth muscle actin | 1A4 | No treatment, 1:1600 | Sigma, St. Louis, Mo |
| Desmin | D33 | No treatment, 1:50 | Dako Corp., Carpinteria, CA |
| S100-protein | polyclonal | No treatment, 1:1600 | Dako |
MW, microwave-based antigen retrieval with citrate buffer; Pepsin, pepsin digestion, 30 minutes at 37°C.