Table 1.
Molecule/antibody | Clone | Pretreatment, Antibody dilution | Source |
---|---|---|---|
CD117 (c-kit) | polyclonal | MW, 1:400 | Santa-Cruz Biotechnology, Santa Cruz, CA |
CD34 | HPCA-1 | Pepsin, 1:50 | Becton-Dickinson, Mt View, CA |
α-smooth muscle actin | 1A4 | No treatment, 1:1600 | Sigma, St. Louis, Mo |
Desmin | D33 | No treatment, 1:50 | Dako Corp., Carpinteria, CA |
S100-protein | polyclonal | No treatment, 1:1600 | Dako |
MW, microwave-based antigen retrieval with citrate buffer; Pepsin, pepsin digestion, 30 minutes at 37°C.