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. 2000 May 16;97(11):5924–5929. doi: 10.1073/pnas.090097797

Figure 3.

Figure 3

Ski represses TGF-β and Smad activity in chimeric and natural PAI-1 promoter-reporters. (A) 3TP-Lux expression in TGF-β-responsive Mv1Lu cells. (B) PAI-1 promoter reporter p800Luc expression in M1vLu cells. (C) TRE-Luc expression in Mv1Lu cells. Cells were transfected with p3TP-Lux, p800Luc, or TRE-Luc reporter plus or minus combinations of Smad2, Smad3, Smad4, Jun, Fos, and c-Ski expression plasmids, as indicated. Luciferase activity, normalized to β-galactosidase activity, was determined as described in Materials and Methods. Where indicated, cells were transferred to medium containing 0.2% FBS plus or minus 1 ng/ml TGF-β, 20 h after transfection. Luciferase activity and β-galactosidase activity were measured 22 h later. The results shown are representative of at least three independent experiments, and data are the average of triplicate measurements plus and minus the standard error.