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. 2000 May 23;97(11):5930–5935. doi: 10.1073/pnas.97.11.5930

Figure 2.

Figure 2

cGMP and NOx production induced by membrane-impermeant E2. (A) HUVEC monolayers were treated with E2hs (equivalent to 10 ng/ml of E2) or E2hsBSA (equivalent to 10 ng/ml E2) in the presence or absence of the ER antagonist ICI 182,780 (10 μM) for 20 min at 37°C, after which cGMP levels were measured by RIA. (B) EA.hy926 monolayers were treated with E2coBSA (equivalent to 10 ng/ml of E2) in the absence or presence of ICI 182,780 for 30 min at 37°C, after which the amount of NOx released into the medium was determined by NO-specific chemiluminescence. *P < 0.01 vs. control.