Figure 3.

brakeless is required in the eye for R1-R6 growth cone targeting. (A and B) Cryostat sections of adult heads carrying the adult R1-R6 marker Rh1-LacZ were stained with anti-β-galactosidase antibody. In the wild type (A), anti-LacZ staining shows R1-R6 cell bodies in the retina and their axons in the lamina. Note that no staining is observed in the medulla. (B) A patch of bks^P1 mutant eye tissue was generated in an otherwise heterozygous or wild-type eye by x-ray-induced mitotic recombination. Some R1-R6 axons migrate into the medulla (arrowheads). (C and D) Third-instar larval eye-brain complexes were stained with mAb 24B10. (C) R-cell projections in a bks⁵ homozygote. (D) R-cell projections in a bks⁵ homozygote carrying a copy of GMR-bks transgene. R-cell termination pattern is very similar to that in the wild type. (Bar = 20 μm.)