Figure 7.
Production of MMP by alveolar macrophages in SP-D (−/−) mice. MMP activity was assessed in macrophage-conditioned media. Pooled alveolar macrophages (1 × 105 cells/well) were incubated in 100 μl of serum-free media for 24 h. The supernatant was collected, concentrated, and analyzed by gelatin zymography. Production of 72-kDa (MMP2) and 105-kDa (MMP9) gelatinases was significantly increased in SP-D (−/−) mice, whereas there was no detectable enzyme activity in SP-D (+/+) mice. Alveolar macrophages were pooled from four SP-D (−/−) mice and eight SP-D (+/+) mice. The assay was performed in three different sets of animals. Data shown are for one assay and are presented as the reverse image of the original zymogram.