Figure 4.

Effect of concentration of the linear DNA cassette on recombination. The strain DY330 was grown at 32°C to OD₆₀₀ = 0.4–0.6, was induced at 42°C for 15 min, and then was made electrocompetent. Different amounts (1, 10, 100, 300, and 1,000 ng) of a linear cat cassette (1 kbp in length) were used to target the cIII kil gam genes of the prophage. Total Cm^R recombinants were plotted vs. the DNA amount at 42°C.