Table 2.

Targeting genes for replacement by the cat cassette

Target Site*	42°C, min	CmR Recombinants†
galK	0	<1
galK	15	2.5 × 104
cIII kil gam	0	<1
cIII kil gam	15	5.0 × 104

^*DY330 competent cells were electroporated with 100 ng of the cat cassette targeted to replace either galK (galK<>cat) or prophage genes cIII kil gam (cIII kil gam<>cat). 

^† Total recombinants per electroporation are shown.