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Genetic requirements on λ
| Strain* | Prophage† | Recombinant‡ |
|---|---|---|
| DY330 | wild-type | 4,100 |
| DY392 | (hin-int)<>amp | 2,000 |
| DY351 | (sieB-kil)<>cat | 4,400 |
| DY386 | (hin-int)<>amp | 1,650 |
| (sieB-kil)<>cat | ||
| DY349 | (gam)<>cat | 0 |
| DY360 | (bet)<>cat | 0 |
| DY359 | (exo)<>cat | 0 |
Competent cells were induced for 15 min and electroporated with 10 ng of linear galK<>tet.
Parenthesis indicate deleted prophage genes (Fig. 1).
Total recombinants per electroporation are shown.
