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PCR analysis of recombination in human cells. A 401-bp product is indicative of site-specific recombination. Lane 1, untransfected pBCPB+; lane 2, in vitro-reacted pBCPB+ (see Materials and Methods); lane 3, Hirt extract (12) containing transfected pBCPB+ from human 293 cells; lane 4, Hirt extract containing transfected pBCPB+ and pCMVInt from human 293 cells; lanes M, size markers.
