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The refre1/372 expression analyzed by RT-PCR. (A) Genomic PCR of DNA prepared from each transformant (lines 7, 8, and 11) and the vector control (VC). (B) Transcript of refre1/372 levels in roots grown under Fe-deficient and Fe-sufficient conditions was detected by using 2 μg of total RNA for each transformant (lines 7, 8, and 11), the vector control (VC), and plasmid containing refre1–372 (P). α-tubulin and OsIRT1 were internal standards.
