Metal concentrations in transformants and vector control grown in calcareous soil. (A–D) Metal concentrations in shoots of transformants (lines 7 and 11, filled bars) and vector control (V, open bars). (E–H) Metal concentrations in seeds of transformants (TF; line 7, filled bars) and vector control (V, open bars).The concentrations of Fe, Zn, Mn, and Cu are expressed as micrograms per gram of dry weight (n = 3). The values followed by different letters are statistically different according to a Student-Newman-Keuls test (P < 0.05).