Figure 5.

Ovarian cancer cell lines DOV13 and TOV112D, neither of which expressed claudin-4, were treated with 5-AZA (1 µmol/l), an inhibitor of DNA methylation. Treatment with 5-AZA resulted in the appearance of new unmethylated MSPCR products (inset) and corresponded with increased expression of claudin-4, as assessed by qRT-PCR (bar graph). The experiments were performed in duplicate. Also shown, MSPCR resulted in a methylated product for the methylated control DNA; PCR products were absent for unmodified DNA (which had not undergone bisulfite treatment). Me, methylated primer; U-Me, unmethylated primer.