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. 2007 Apr;9(4):304–314. doi: 10.1593/neo.07118

Figure 6.

Figure 6

(A) The schematic diagram represents claudin-4 mRNA: +1, transcription initiation site; | (vertical marks), CpG dinucleotide; blue band, CpG island identified using CpG Island Searcher software (http://ccnt.hsc.usc.edu/cpgislands/) and the displayed criteria; → (arrowhead), forward (BSF, bp 156–180) and reverse (BSR, bp 468–491) primer locations for the bisulfite sequencing; ▸ (arrows), forward (MSPF, bp 233–256) and reverse (MSPR, bp 432–452) primer locations for MSPCR. (B) The ovarian cancer cell line OVCA429, which exhibited a hypomethylated CLDN4 allele only (per Figure 4B), underwent bisulfite sequencing. Complete hypomethylation was demonstrated at all 21 CpG dinucleotides (circled and numbered) in the sequenced fragment.