Figure 4.

Effect of MINA-05 pretreatment on colony formation of PC cell lines in vitro. LNCaP-FGC and PC-3 cells were treated with MINA-05 at ½ x IC₅₀, IC₅₀, or 2 x IC₅₀ concentrations, or 0.5% DMSO (vehicle), in triplicate. After 72 hours, cells were harvested, cell viability was determined by trypan blue exclusion assay, and viable cells were seeded for clonogenic assays (A) in soft agar and (B) on plastic. (C) Total number of colonies (>50 cells) was counted by using an inverted light microscope (original magnification, x38.4). Graphs indicate mean ± SD of three independent experiments. *P < .05, **P < .01 relative to vehicle.