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Proliferation and differentiation are reduced in E15 CXCR4-/- cortical cultures. (a, b) Graphs show diminished proliferation as evidenced by Ki67 immunolabeling (a) and increased cell death quantified by propidium iodide or trypan blue staining (b) in mutant cultures compared to wild type controls. (c, d) Staining of cultures for Neu-N also showed fewer postmitotic neurons (c) and GABAergic neurons (d) in mutant cultures compared to wild type controls.
