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. 2007 Apr 12;6:11. doi: 10.1186/1475-925X-6-11

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Copyright © 2007 Narkilahti et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of hESC colonies cultured in conventional hESC medium: (a) A representative growth graph of an HS293 colony cultured using the automated culture platform for 7 d after passaging. The growth rate of the colony was most rapid from day 5 onwards. An HS293 colony at day 1 (b), day 3 (c), and day 7 (e) after passaging, monitored using the automated system. (d) FACS analysis of HS237 colonies at day 7 after passaging. The red column represents the negative control sample and the white column represents SSEA4-positive cells (81%). An HS293 colony monitored with the automated system was immunohistochemically characterized (f) using an antibody against an undifferentiated hESC marker, Nanog (green), and a differentiated hESC marker, SSEA1 (red). Scale bar 200 μm (b,c,e), 100 μm (f).