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PCR analysis demonstrating Cre-mediated recombination in DNA isolated from kidney (Ki), liver (Li), heart (He), lung (Lu), and cell cultures derived from heart (cHe) and lung (cLu) from Sm22-Cre+/0;Smad5fl/− and Tie-1-Cre+/0;Smad5fl/− adult mice. M, marker, 1-kb DNA ladder. Two primer sets were used: a CreFW and CreREV primer (top) were used for amplification of the 400-bp Cre fragment, primers 1 and 3 for the recombined 387-bp Smad5 fragment only.
