Figure 4.

In vitro H/R stimulates apoptosis; the addition of vitamins or the p38 inhibitor suppress the effects of H/R. Protein lysates from placentas cultured under H/R in the presence or absence of vitamins C and E (V) or PD169316 (P-I) for 7 hours (n = 6) or 16 hours (n = 6) were analyzed for cleaved caspase-3 (undetectable at 7 hours) (a) and cleaved caspase-9 (b). Representative blots show the 16-hour time point. *P < 0.05 compared with normoxic control; ^#P < 0.05 compared with H/R treatment.