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. 2007 May;170(5):1594–1606. doi: 10.2353/ajpath.2007.061025

Figure 5.

Figure 5

Induction of VEGF expression by DLX4. A: Levels of VEGF in culture supernatants of A2780 (i) and ES-2 (ii) cell lines at 24 hours after seeding, as measured by ELISA and normalized to the total cellular protein content, and in ascites (iii) collected from mice at 18 days after inoculation with the indicated stably transfected ES-2 cell lines. B: Western blot of DLX4 expression in A2780 DLX4-2 cells and in ES-2 DLX4-10 cells, after treatment with DLX4-specific siRNA, negative control siRNA (neg.) and with no siRNA (mock). C: Levels of VEGF in supernatants of A2780 DLX4-2 (i) and ES-2 DLX4-10 (ii) cells after mock and siRNA treatment.