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Dual-fluorescent immunostaining of ABCG2 and M3R in normal liver, dysplastic nodule, and HCC. In normal liver, bile ducts coexpressed ABCG2 (green) and M3R (red). Coexpression of ABCG2 and M3R is commonly observed in periportal hepatocytes in dysplastic nodule and scattered cancer cells in HCC (arrows). Original magnifications: ×400 (normal liver and HCC); ×200 (dysplastic nodule).
