Table 2.
Sequences, Annealing Temperatures, Cycle Times, and Product Sizes of PCR Primers
Molecule | F/R | Sequence | Temperature (°C) | Cycle | Product size (bp) |
---|---|---|---|---|---|
ABCG2 | F | 5′-CACCTTATTGGCCTCAGGAA-3′ | 52 | 35 | 209 |
R | 5′-CCTGCTTGGAAGGCTCTATG-3′ | ||||
M3R | F | 5′-CCTTCAAGGAAGCCACTCTG-3′ | 52 | 35 | 219 |
R | 5′-AGCCCAGATTCCAAAAGGTT-3′ | ||||
CK19 | F | 5′-TCCCGCGACTACAGCCACTACTACACGACC-3′ | 52 | 35 | 396 |
R | 5′-TTGGCTTCGCATGTCACTCAGGAT-3′ | ||||
c-kit | F | 5′-AAAGTATAGGTTTAGCCTCCTTCGCA-3′ | 54 | 35 | 608 |
R | 5′-CAAATGGTTACTTCCAGATAACGGC-3′ | ||||
CD34 | F | 5′-CACCCTGTGTCTCAACATGG-3′ | 51 | 33 | 190 |
R | 5′-GGCTTCAAGGTTGTCTCTGG-3′ | ||||
CD56 | F | 5′-TCCATCACCTGGAGGACTTC-3′ | 52 | 37 | 210 |
R | 5′-CTTTGGGGCATATTGCACTT-3′ | ||||
CK14 | F | 5′-TTCTGAACGAGATGCGTGAC-3′ | 53 | 35 | 224 |
R | 5′-GTTCTCCAGGGATGCTTTCA-3′ | ||||
AFP | F | 5′-GGGAGCGGCTGACATTATTA-3′ | 52 | 37 | 231 |
R | 5′-TCTTGCTTCATCGTTTGCAG-3′ | ||||
Albumin | F | 5′-TGCTTGAATGTGCTGATGACAGGG-3′ | 52 | 37 | 161 |
R | 5′-AAGGCAAGTCAGCAGGCATCTCATC-3′ | ||||
GATA6 | F | 5′-GCCAACTGTCACACCACAAC-3′ | 53 | 35 | 216 |
R | 5′-TGGAGTCATGGGAATGGAAT-3′ | ||||
C/EBPα | F | 5′-TCACCGCTCCAATGCCTAC-3′ | 53 | 35 | 210 |
R | 5′-CCCTATGTTTCCACCCCTTTC-3′ | ||||
C/EBPβ | F | 5′-CTTCAGCCCGTACCTGGAG-3′ | 53 | 35 | 136 |
R | 5′-GGAGAGGAAGTCGTGGTGC-3′ | ||||
β-Actin | F | 5′-CAAGAGATGGCCACGGCTGCT-3′ | 55 | 25 | 334 |
R | 5′-TCCTTCTGCATCCTGTCGGCA-3′ |
F, forward; R, reverse.