Figure 5.

IL-2Rα exhibits submicron-scale colocalization with MHC II but not with TrfR. A representative confocal fluorescence image of the colocalization of IL-2Rα and HLA class II is shown in a. IL-2Rα and HLA class II are labeled with XF (green) and XR (red), respectively. Because of the high degree of colocalization, many pixels appear orange when the two channels are fused. b demonstrates that IL-2Rα (green) and TrfR (red) codetected in a similar experiment are mostly localized at different areas of the plasma membrane. Bar = 2 μm.