Figure 2.

Specificity of a novel polyclonal Rfp2 antibody. (A) To monitor the specificity of the Rfp2 antibody, plasmids expressing FLAG-tagged Rfp2 were cotransfected with siRNA oligos targeting RFP2 and DLTET, respectively. Blots were developed using Rfp2-specific polyclonal antibodies to detect exogenous Rfp2 protein. (B) Extracts of HEK293 cells treated with RFP2 siRNA oligos, and siRNA oligos targeting green fluorescent protein (GFP) were analyzed for endogenous Rfp2 expression by Western blotting with Rfp2 antibody. Numbers indicate protein size in kDa. Detection of tubulin was used as loading control.