Figure 8.

Effect of Kifc1 antibody on directional motility of mouse liver early endocytic vesicles. Fluorescent ASOR-containing endocytic vesicles from wild-type mouse liver were bound to polarity marked microtubules in an optical chamber. Vesicles were incubated for 6 min in the absence or presence of Kifc1 antibody. The bars indicate the percentage of motile vesicles moving toward the plus- (black) or minus (gray)-ends of microtubules after addition of 50 μM ATP. Motility of 36 vesicles was examined in buffer and 42 in the presence of Kifc1 antibody over five independent experiments for each condition. *p < 0.05 compared with control by chi-square analysis.